Trichoderma species are efficient bio-control factors due to their resistance to pathogenic fungi on plants in various mechanisms. One of these is the production of a large variety of cell-wall degrading enzymes, especially chitinase enzymes. This kind of enzyme has a highly chitinolytic ability of breaking down the chitin that is responsible for rigidity on cell wall of several plant pathogens. In this study, the authors maximized the transplanting conditions for highly active chitinase from Trichoderma sp., which serves as a base for creating chitinase productions applied in protecting plants from diseases.