Pangasius krempfi does not reproduce naturally in captivity. Therefore the use of hypophysaqon is required. For females, several injections were used, including the first four injections with 500 IU of HCG (Human Chorionic Gonadotropin) per kg or pituitary gland extract of common carp (3 kg donor fish used for 8 kg receiver), preliminary one injection of 1,500 IU of HCG per kg, followed by a final injection of 6,000 IU of HCG per kg. For males, only one injection of 2,000 IU of HCG per kg was applied at the time of final injection for female. The females were stripped for eggs from 9.5 to 16.5 hours after the final injection. Fertilization rate varied from 0 to 96.4 percent (an average of 54.07+/- 53.63 percent). Hatching took approximately 24 to 36 hours at water temperatures of 27 - 30°C. Hatching rate was found to be at 0 - 93.0 percent (an average of 51.88 +/- 30.92 percent).