Prunus mandshurica (Maxim.) Koehne pulp and almonds are rich in nutrients and bioactive compounds
however, their chemical composition and content remain unexplored. We systematically evaluated their physicochemical properties and antioxidant capacities using 14 clones. Significant differences in physicochemical properties observed between the tested clones provided opportunities for developing and utilizing germplasm resources. Clones with thicker pulps exhibited sweet flavors, heavier fruits, lighter almonds, and lower kernel rates. Their pulp was relatively low in total sugar (1.66 - 14.91%) and soluble solids content (6.23 - 18.67°Brix) but rich in titratable acidity (2.81 - 4.20%) and vitamin C (0.97 - 1.65 mg/g), making them a promising option for food and nutraceutical applications. Almond oil was the main component (50.02%), followed by protein (25.82%), and amygdalin (3.17%), with high balanced amino acid levels. Nine clones with amygdalin content >
3% indicated a high medicinal value. The fruit was rich in total phenolics, total flavonoids, and antioxidant capacity, and its pulp exhibited a stronger antioxidant capacity than that of almonds. Clones 783 and 717 had sweet and thick pulp with high nutrient content and antioxidant capacity, whereas clone 772 pulp exhibited a strong antioxidant capacity and a high yield, making it a good source of natural antioxidants. We are the first to assess the physicochemical properties and antioxidant capacity of 14 P. mandshurica clones fruit to explore their potential applications in the food and pharmaceutical industries, and provide data to support the selection of excellent germplasm for different needs.