This review article focuses on efficient strategies for detecting food contamination, particularly through the use of nanobiosensors. It begins with an overview of biosensors, highlighting their basic components and underlying mechanisms. The article then explores the advancements driven by nanotechnology, specifically examining key nanomaterials-such as nanoparticles, nanowires, and nanotubes-that play a crucial role in sensor development. A comprehensive analysis of the diverse applications of nanobiosensors in the food industry follows, including their use in detecting foodborne pathogens, toxins, and other harmful contaminants. The integration of nanobiosensors with Internet of Things technologies is also discussed, emphasizing the potential of smart packaging solutions for real-time monitoring and data analysis. The article critically assesses the challenges and prospects of nanobiosensor applications, addressing issues such as sensor sensitivity, specificity, cost-effectiveness, and regulatory concerns. Furthermore, emerging trends and future directions are explored, particularly the shift toward more sustainable and eco-friendly sensor technologies. The review concludes by emphasizing the transformative impact of nanobiosensors in enhancing food safety, quality control, and innovation across the food industry. By offering a thorough examination of current technologies and potential future developments, this article aims to contribute to the continued evolution of nanobiosensor applications in food safety.