Oligonucleotides are essential for gene regulation, expression, and disease biomarker identification, yet their small size presents challenges due to lower abundance and increased susceptibility to degradation in biological samples. Addressing these challenges, a novel approach was developed for effective oligonucleotide extraction, consisting of a commercially available nitrocellulose (NC) membrane non-covalently modified with a combination of single-walled carbon nanotubes (SWCNTs) and polyethylene glycol (PEG) aminated reduced graphene oxide (GA). The membrane was evaluated for the extraction of a fluorescent labelled single-stranded deoxyribonucleic acid (ssDNA), with fewer than 30 nucleotides, from complex solutions containing various ionic species (MnCl