The objective of this study was to detennine some characteristic physiological and biochemical changes of lychee fruits in controlled atmosphere (CA) environment, as a basis for building the process of lychee preservation technology by CA The research content of subject "Research on technoloy and design manufacturing CA equipment systems to apply for vegetabales, fruits and flowers storage at Ministry level (2011 -2013)". Experiments have been on Thanh Ha lychee which were grown in Luc Ngan, Bac Giang provine. The experimental single and multi-element have been done on laboratory CA equipment system which is the product of the subject. The CA equipment system can be control four paratneters with high accuracy (as temperature +/- 0.5°C, relative humidity +/- 2 percent, O2 concentration +/- 0.5 percent, CO2 concentration +/- 0.5 percent). From the optimization results have determined control atmosphere of 4.21 +/- 0.5 percent O2 + 7.0 +/- 0.5 percent CO2 at 4.35 +/- 0.5°C, with 90 +/- 2 percent relative humidity. After 35 days store in the CA, the quality of Luc Ngan lychee achived the requirement of sensory quality, nutrition quality, and the capable of maintaining commercial quality in cold storage conditions 4°C for 3-5 days.