Tạo dòng và biểu hiện gen mã hóa protein cp23 từ cryptosporidium parvum

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Tác giả: Thị Bích Lân Đinh, Tấn Quảng Hoàng, Quốc Việt Lê, Hoàng Lộc Nguyễn, Văn Quyết Phan, Thăng Long Phùng, Thúy Lan Trần

Ngôn ngữ: vie

Ký hiệu phân loại: 593.99 *Hemichordata

Thông tin xuất bản: Nông nghiệp & Phát triển Nông thôn, 2014

Mô tả vật lý: 59-64

Bộ sưu tập: Metadata

ID: 567342

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The conducted cloning the cp23 gene coding Cp23 (glyco) protein from Cryptosporidium parvum (C parvum) isolated from cattle in Thua Thien - Hue province, Vietnam. The coding region of cp23 gene from C
palVUm is 99 percent (nucleotide) and 100 percent (amino acid) similarity with cpP23 gene deposited in NCBI (accession number: U34390). SDS-PAGE and Western blot analysis showed that the cp23 gene in E coli BI21 StarTM(DE3) produced polypeptides with molecular weights of approximately 37 and 49 kDa. These molecules may be non- or glycosylated P23 fusion polypeptides. Western blot result showed that both proteins had reaction with monoclonal antibody against Cp23 antigen. Recombinant Cp23 protein purified by GST (glutathione S-transferase) affinity chromatography can be used as an antigen for vaccine or antibody production as well as for development of diagnostic kit for C parvum.

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