Neocarzinostatin (NCS) is an antibiotic that belongs to the natural enediyne antibiotics, extracted from Streptomyces carzinostaticus ATCC 15944. NCS structure is very unstable but shows strongly biology. Because of itself toxic makes the cultural bacteria changed and causes the yield of NCS low. The authors studied the optimal culture for Streptomyces carzinostaticus ATCC15944. From 4 cultured media: R2, R2YE, YEME and N-Z amin the authorsfound that the N-Z amin medium is the best medium for culturing Streptomyces carzinostaticus ATCC 15944. In addition, at the 72h of culturing,the biomass and biological showed the highest.