The microbial diversity of razor clam in refrigerator temperature was investigated to identify the spoilage bacteria. The bactericidal efficiency and mechanism of SAEW on the P. fluorescens based on the cell-level detection indexes, and motion characteristics were investigated. The bacterial community structure of razor clam during storage as determined by high-throughput sequencing technology demonstrated that Vibrio corresponding to the phylum Proteobacteria were the dominant genera at D0 group
Through dynamic changes in the microbial community during refrigerated storage, Pseudomonas gradually becomes the dominant species, demonstrating strong adaptability. Furthermore, a significant antibacterial effect of SAEW on P. fluorescens has been observed (P <
0.05), with a 30 ppm SAEW treatment for 60 s reducing P. fluorescens by 3.85 log CFU/ml. Under these treatment conditions, the inhibition rate of cellular viability of P. fluorescens was 56.66 %, the inhibition rate of biofilm formation was 84.59 %, the increase rate of AKP activity was 48 %, the nucleic acid leakage increased by 49.00 %, the protein leakage increased by 1.64 times, and the inhibition rates of swarming, swimming and twitching motility reached 76.05 %, 70.97 % and 93.57 % respectively. The results indicated that SAEW significantly damaged the cell surface structure of P. fluorescens and had a significant inhibitory effect on bacterial motility. Therefore, the cell wall and membrane were the direct targets of SAEW, and their damage was the direct cause of inactivation of P. fluorescens. In summary, this study provides a theoretical foundation for the development and application of novel preservation technologies based on SAEW technology.