With an increase in awareness of health, environmental conservation and animal welfare, the market for plant proteins is expanding. However, the low solubility and poor functional properties of plant proteins near the isoelectric point limit their application in food processing. Glycosylation refers to the structural modification of proteins by introduction of polysaccharides to form protein-polysaccharide conjugates in the early stages of Maillard reaction. Glycosylation is a green and efficient method that has been proved to produce modified proteins with superior solubility, emulsifying and forming properties. Glycosylation and the application of protein-carbohydrate conjugates have become research hotspots in recent years. This paper presented a comprehensive review of the effects of glycosylation on the functional properties of plant proteins and the mechanisms of non-thermal physical treatments assisted glycosylation. It was demonstrated that glycosylation modified the structure of plant proteins and improved their functional properties. Non-thermal physical treatments assisted glycosylation increased the reactive sites of plant proteins and further improved their functional properties. Protein-carbohydrate conjugates could be applied in delivery systems, films, emulsifiers and other applications, which have significant research prospects in food applications.