Stability and activity of TG25P phage in control of Aeromonas hydrophila in striped catfish pond water = Stability and activity of TG25P phage in control of Aeromonas hydrophila in striped catfish pond water

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Tác giả: A. Hoang Hoang, D. Tam Le, Xuan T.T. Tran

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Science and Technology Development Journal, 2018

Mô tả vật lý: tr.64

Bộ sưu tập: NCBI

ID: 577124

Striped catfish (Pangasianodon hypohthalmus) is a native freshwater fish species inthe Mekong Delta, Vietnam, and significantly contributes to national aqua exports. Currently, however,the sustainable development of striped catfish farming is being affected by bacterial pathogeninfections, of which hemorrhagic septicemia caused by Aeromonas hydrophila bacteria is one of themost common diseases. Methods: In this study, the stability of TG25P and CT45P phages to factorssuch as temperature, pH, and organic solvents was investigated, with the stability of TG25P beingfound to be higher than that of CT45P. Results: The activity of TG25P was retained to approximately90% and 80% at 37oC and 50oC for 1 h, respectively. Its activity was maintained to greaterthan 80% at pH 5-9 for 24 h and approximately 90-100% in organic solvents, such as chloroform ordiethyl ether, for 1 h. In addition, the stability and activity of TG25P for the control of A. hydrophila instriped catfish pond water was also evaluated for 48 h. Conclusion: TG25P was found to be highlyapplicable in the creation of low-cost phage-containing products for the prospective applicationof phage therapy in prevention and treatment of hemorrhagic septicemia in striped catfish.
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