A Cross sectional descriptive study was carried out of 53 infants aged 0-28 days who were diagnosed of neonatal sepsis, treated at the Neonatal intensive care unit of Can Tho Children's Hospital from 4/2016 to 6/2017. All patients, there were 54.7% of male and 45.3% of female, of them 28.3% was premature and low birth weight children. The clinical and paraclinical characteristics of neonatal sepsis were: fever 69.7%, hypothermia 13.2%, tachypnea 81.1%, tachycardia 20.8%, bradycardia 13.2%, leukocytosis 52.8%, increased CRP 57.7%, infitration on lung X-ray 88,7%. Gram-negative 64.2%, Gram-positive 35,8% (Staphy 35,8%). Early neonatal infection 50.9%, late neonatal infection 49.1%. Bacteria causing early neonatal infection were Kleb, E. coli and Acineto. Bacteria causing late eonatal infection were Staphy and Pseudo. Gram-negative bacteria were susceptible to Tienam, resistant to Ampicillin and Cefotaxim. Gram positive bacteria were susceptible to Tienam, Vancomycin and resistant to Oxacillin, Ampicillin. The survival rate was 84.9%, mortality rate was 15.1%.