AST, ALT tests represent for liver diseases, Glucose test represents for diabetes, Total Protein represents for diseases of kidney function, Cholesterol and Triglycerides tests represent for diseases of duslipidemia and atherosclerosis [1,2,3]. The study aimed to identify standard analytical equipment and methods for 6 parameters based on reference methods and reference materials. The research results determined that the standard method for testing AST, ALT is Trisbuffer without pyridoxal-5-phosphate, Glucose is Glucose oxidase, Total protein is Biuret reaction, Cholesterol is Cholesterol oxidase, Triglyceride is Lipase/GPO-PAP no correction, the equipments were Abbott, Beckman Coulter, Erba