Optimum conditions for transformation reaction of some substituted benzaldehyde 4-(2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl)thiosemicarbazones 1 with ethyl bromoacetate were investigated. The optimum conditions were the use of absolute ethanol as solvent, anhydrous sodium acetate as catalyst and 10 min irradiation in MW oven after stirring the reaction mixture in room temperature for 30 min. Products, 2-iminothiazolidin-4-ones 2/2, were formed in 75-86 percent yields, as pair of isomers in ratio determined by NMR spectra. Structures of these 2-iminothiazolidin-4-ones were confirmed by IR, NMR and mass spectra.