Introduction: In vitro cultivation of DCs and cytokine-induced killer cells (CIK cells) — a specialphenotype of T lymphocyte populations — for cancer treatment has gained significant researchinterest. The goal of this study is to understand whether the priming from DCs helps CIK cells toexert their toxic function and kill the cancer cells. Methods: In this research, DCs were differentiated from mononuclear cells in culture medium supplemented with Granulocyte-macrophagecolony-stimulating factor (GM-CSF), and Interleukin-4 (IL-4), and were induced to mature with cancer cell antigens. Umbilical cord blood mononuclear cells were induced into CIK cells by Interferonγ (IFN-γ), anti-CD3 antibody and IL-2. After 4-day exposure (with DC:CIK = 1:10), DCs and CIK cellsinteracted with each other. Results: Indeed, DCs interacted with and secreted cytokines that stimulated CIK cells to proliferate up to 133.7%. In addition, DC-CIK co-culture also stimulated strongexpression of IFN-γ. The analysis of flow cytometry data indicated that DC-CIK co-culture highlyexpressed Granzyme B (70.47% ± 1.53, 4 times higher than MNCs, twice higher than CIK cells)and CD3+CD56+ markers (13.27% ± 2.73, 13 times higher than MNCs, twice higher than CIK cells).Particularly, DC-CIK co-culture had the most specific lethal effects on cancer cells after 72 hours.Conclusion: In conclusion, co-culture of DCs and CIK cells is capable of increasing the expressionof CIK-specific characteristics and CIK toxicity on cancer cells.