Phân lập, định lượng các thành phần thu được trong mẫu keo ong từ rừng nhiệt đới Việt Nam = Isolation, quantification of the active pharmaceutical compounds in propolis in Vietnam’s rainforests

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2019

Mô tả vật lý: tr.60

Bộ sưu tập: NCBI

ID: 582097

Thêm vào giỏ

Propolis is a natural resin, collected by honeybees (Apis mellifera) from buds of different plants, added with beewax and salivary secretions, in order to repair and disinfect the beehive. Firstly, the diversity of propolis in tropical areas led us to explore the chemical compositions of Vietnam propolis, especially five samples from Eawy. The one from Europe had an unusual chemical composition (flavonoids, phenolic acids and esters) associated with a “polar” type propolis. The five other propolis extracts showed the presence of major apolar compounds and different minor polyphenols. Materials and methods: A fractionation by Flash chromatography of the ethanolic extract of Eawy propolis resulted in14 fractions, 3 of which were analysed by dereplication (HPLC/UV and HPLC/MS). Results: One of the major compounds of this kind of propolis was purified and analysed by proton NMR. It is a flavanone namely pinocembrine. Tình hình chung và mục tiêu nghiên cứu: Keo ong là một loại nhựa tự nhiên, được thu thập bởi ong mật (Apis mellifera). Ong mật sẽ thu thập keo ong từ chồi của các loại cây khác nhau. Với sự đa dạng của keo ong ở vùng nhiệt đới, nhóm nghiên cứu tiến hành xác định thành phần hóa học của keo ong rừng Eawy (Đắk Lắk) so sánh với một mẫu keo ong châu Âu và một mẫu keo ong thương mại. Mẫu keo ong châu Âu có thành phần hóa học là flavonoid, acid phenolic và este liên quan đến keo ong loại poplar. Và 5 chiết xuất keo ong khác từ rừng Eawy cho thấy sự hiện diện của các hợp chất và các polyphenol khác nhau. Phương pháp nghiên cứu: Phân tích bằng sắc kí flash của dịch chiết etanolic thu được 14 phân đoạn, 3 trong số đó được phân tích bởi HPLC/UV và HPLC/MS, NMR. Kết quả : Một trong những hợp chất chính của keo ong này đã được tinh chế và được phân tích bằng NMR. Đó là một flavanone cụ thể là pinocembrine.

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