Ảnh hưởng mãn tính của phơi nhiễm Bisphenol A lên cá sọc ngựa (Danio rerio) ở giai đoạn Juvenile=Chronic efffects of bisphenol an exposure on zebrafish (Danio rerio) at juvenile state

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Thông tin xuất bản: Tạp chí Phát triển Khoa học & Công nghệ: Khoa học Trái đất và Môi trường, 2019

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Bisphenol A (BPA) is an endocrine disrupting chemical, therefore some countries have banned the use of it in plastic containers for food storage and plastic toys for children. BPAcan be found in natural waters with measured concentrations varying from ng/l to mg/l. However, in fact that the chronic ecotoxicology data obtained from aquatic animals for BPA exposure at these levels are quite few. Methods: In this study zebrafish at 30 days of age were exposed to four variants0, 1, 10 and 100 μg/l BPA along 60 days, which is the fasted growth period in zebrafish (Juvenile stage). At the end of the experiments, all fish weight and length were measured and their fresh livers were used for histological assessment. Results: The results showed that weight and length of fish in 100 mg/l BPA group increased significantly as compared with that in the other experimental groups. Also only in this group, changes in hepatic morphology such as vacuolar number and sizeincreasement, cytoplasmicand/or nuclear enlargement, glycogen reduction, lipid enhancement, were observed, but especially no significant inflammation. Fish in the other groups exposed to BPA concentrations <
100mg/l had no change in all targeted endpoints as compared with that of the control. Conclusion: Chronic exposure of 100mg/L BPA to zebrafish along the fast-growth period (Juvenile) caused increasement of weight and length, and altered morphology of hepatic cells, thatmay harm to liver functionsBisphenol A (BPA) là chất gây rối loạn nội tiết tố, vì vậy một vài quốc gia cấm sử dụng chất này trong bao bì nhựa đựng thực phẩm và đồ chơi nhựa cho trẻ em. BPA được tìm thấy trongnước mặt ở nồng độ đo được từ ng/L- mg/L. Tuy nhiên các dữ liệu độc học về phơi nhiễm mãn tính động vật thủy sinh với BPA ở dải nồng độ này hiện tại rất ít. Phương pháp: Nghiên cứu này sử dụng cá sọc ngựa 30 ngày tuổi, cho phơi nhiễm với BPA 0, 1, 10 và 100 μg/L, trong 60 ngày, đây là giai đoạn tăng trưởng mạnh (Juvenile). Kết thúc thí nghiệm, tất cả cá được đo chiều dài, cân trọng lượng, lấy mô gan tươi để đánh giá bệnh học. Kết quả: Chiều dài và trọng lượng cá tăng hơn ở nhóm phơi nhiễm với BPA 100 μg/L so với các nhóm khác. Cũng chỉ ở nhóm này quan sát thấy sự thay đổi hình thái tế bào gan như tăng số lượng và kích thước không bào, trương nở nhân và tếbào chất, giảm glycogen và tăng lipid, nhưng không có hiện tượng viêm. Cá trong các nhóm phơi nhiễm với nồng độ BPA <
100 mg/L cho thấy các điểm cuối đáp ứng không có sự khác biệt so với nhóm đối chứng. Kết luận: Phơi nhiễm mãn tính với BPA 100 mg/L lên cá sọc ngựa ở giai đoạn Juvenile gây tăng trọng lượng, chiều dài làm thay đổi hình thái tế bào gan, có thể gây hại lên chức năng gan

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