Microfluidic Chip for Studying the Mechanisms of Allogeneic Progenitor Neuronal Cells Integration into a Mature Neural Network In Vitro.

• Tác giả: A A Gladkov, V B Kazantsev, V N Kolpakov, I V Mukhina, Y I Pigareva, A S Pimashkin, M S Zemlyanskov
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Bulletin of experimental biology and medicine , 2025
• Bộ sưu tập: NCBI
• ID: 58862

Tóm tắt

The formation of functional connections between cells derived from neuronal progenitor cells (NPCs) and the developed neuronal network is a crucial task in neurobiology and cellular technologies for medical applications. We developed a technique to co-culture NPCs and mature neuronal cells in a three-chamber microfluidic chip in vitro and studied the features of functional connections among cells derived from NPCs during their integration into a network of differentiated neurons. Starting from day 20 of primary culture development, a network forms from integrated NPCs, which ensures the distribution of bioelectrical activity among neurons in a manner similar to that of differentiated cells.