OBJECTIVE: To evaluate the diagnostic performance of dual-time-point METHODS: Twenty treatment-naïve patients with histology-proven PCa who underwent RESULTS: Lesion detection was consistent at both 30 and 60-min p.i. imaging of 20 patients (mean age 72 ± 9), identifying 27 primary prostate lesions, 84 lymph node metastases, bone metastases in 8 patients, and other metastases in 2 patients. Primary prostate lesions showed no significant difference in SUVmax and target-to-blood pool (T/B) ratios between the two imaging times, while these parameters significantly increased over time in bone metastases. Lymph node metastases showed no significant difference in SUVmax but higher T/B ratios at 60 min compared to 30 min. A higher frequency of UBU was observed at 60 min (37.3%) compared to 30 min (32.3%), with significantly higher SUVmax and T/B ratios at 60 min. 85.6% UBU was categorized as PSMA-RADS 2 at 60 min, and the others were PSMA-RADS 3 or 4. The most frequent localization was vertebrae, followed by ribs. Interrater reliability was almost perfect for lesion detection at both time points. CONCLUSION: Early 30-min