BACKGROUND: Light at night (LAN) has become a global concern. However, little is known about the effects of bedroom LAN exposure on glucose metabolism markers. We aimed to explore the association between intensity and duration of bedroom LAN exposure with glucose metabolism markers, and the role of circadian-dependent meal timing in these associations. METHODS: Real-ambient bedroom LAN exposure was measured using a portable illuminance meter for two consecutive days. Seven consecutive days of sleep time and 24-h dietary records were assessed by wrist-worn accelerometer and mobile phone photos, respectively. Circadian-dependent meal timing, including the timing of meal relative to clock time and sleep time, was calculated from 24-h dietary records and sleep data. RESULTS: The mean age of participants was 18.7 years, and 32.8 % were male. Per SD lx increase of bedroom LAN CONCLUSIONS: Overall, bedroom LAN exposure was associated with impaired glucose metabolism markers among young adults. Importantly, circadian-dependent meal timing may have potentially moderate effects on these associations. Keeping bedroom darkness at night and adhering to early eating pattern may be important public health strategies to reduce the risk of glucose metabolism disorders.