Impedance-based method for the quantification of infectious SARS-CoV-2.

• Tác giả: Christophe Cordevant, Audrey Fraisse, Laurent Guillier, Sophie Le Poder, Sandra Martin-Latil, Sylvie Perelle
• Ngôn ngữ: eng
• Thông tin xuất bản: Netherlands : Journal of virological methods , 2025
• Bộ sưu tập: NCBI
• ID: 61196

Tóm tắt

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent involved in the coronavirus disease 2019 (COVID-19) pandemic. The development of infectious titration methods is crucial to provide data for a better understanding of transmission routes, as well as to validate the efficacy of inactivation treatments. Nevertheless, the low-throughput analytical capacity of traditional methods may be a limiting factor for a large screening of samples. The aim of the study was to develop a Real-Time Cell Analysis (RTCA) assay based on the measurement of cell impedance to quantify infectious SARS-CoV-2. The kinetics of cell impedance showed a virus-specific Cell Index (CI) drop. This enabled the correlation between viral concentrations and time at which a 50 % drop in CI values was observed (tCI