ETHNOPHARMACOLOGICAL RELEVANCE: Taxus globosa Schltdl. (Taxaceae) is commonly named "Tejo mexicano". It's a Mexican plant known in folk medicine as a remedy for pain such as stomachache and headache, arthritis, gout, and other inflammatory conditions. It belongs to the Taxus genus and contains one of the most successful natural anticancer drugs, paclitaxel, among other bioactive compounds. OBJECTIVE OF THE STUDY: To provide preclinical evidence of its phytochemical, toxicological, and pharmacological properties at central and peripheral levels for analgesia, as well as anxiolytic, due to its central nervous system (CNS) depressant properties. MATERIAL AND METHODS: Initially, the median lethal dose (LD RESULTS: The acute toxicity was estimated to be greater than 316.23 mg/kg, i.p. in mice. The T. globosa extract produced a significant anxiolytic effect at 30 mg/kg, i.p., and an antinociceptive effect at a dosage of 56.2 mg/kg, i.p., mainly as anti-inflammatory, where both endogenous opioids and 5-HT CONCLUSION: Our results provide preclinical evidence for the pharmacological medicinal properties of T. globosa in producing CNS depressant activity, useful as a remedy for anxiety and pain therapy in folk medicine.