Evaluation of a surrogate virus neutralization assay for detecting neutralizing antibodies against SARS-CoV-2 in an African population.

• Tác giả: Emmanuel Balandya, Godfrey Barabona, Frank Eric Hassan, Doreen Kamori, Benson Kidenya, Juma Kisuse, Ponsian P Kunambi, Eligius Lyamuya, Salim Masoud, Sayoki Mfinanga, Frank Msafiri, Isaac Ngare, Lilian Nkinda, Emmanuel Nkuwi, Elisha Osati, Mbazi Senkoro, Victoria Shayo, Takamasa Ueno
• Ngôn ngữ: eng
• Ký hiệu phân loại: 304.6 Population
• Thông tin xuất bản: England : Biology methods & protocols , 2025
• Bộ sưu tập: NCBI
• ID: 61385

Tóm tắt

 The global resurgence of coronaviruses and the move to incorporate COVID-19 vaccines into the expanded program for immunization have warranted for a high-throughput and low-cost assay to measure and quantify mounted neutralizing antibodies as an indicator for protection against SARS-CoV-2. Hence, we evaluated the surrogate-virus-neutralization-assay (sVNT) as an alternative assay to the pseudo-virus neutralization assay (pVNT). The sVNT was used to measure neutralizing antibodies among 119 infected and/or vaccinated blood samples, against wild-type SARS-CoV-2 (WT) and the Omicron-variant with reference to the pVNT. Four different cut-offs were assessed for suitability in distinguishing neutralizers: the manufacturer (>
 30%), literature-based (>
 50%) and (>
 80%), and population-based (>
 27.69%). The obtained data was analyzed using "R" through its integrated development environments
  JAMOV and R-Studio. Using the WT strain, only the population-based cut-off was able to differentiate neutralizers from non-neutralizers beyond chance, with an area under the curve (AUC) of 0.833 (95%CI, 0.505-1.0