In this study, the policistron of idi, crtE, crtI and crtB based on pRSET-A vector were successfully constructed to harbour idi, crtE, crtI abd crtB. Overexpression of the policistron under the control of T7 promotor generated red cell pellet. HPLC analysis of the 24h culture confirmed the availability of lycopene at 1.15 mg/l.