The nucleotide sequence of 645bp fragments on 16S rRNA of 5 C. perfringens strains isolated from diarrhea-enteritis goats and sheep reared in Vietnam were completely identical
they were highly similarity (99,8 - 100 percent)
in which the 16S rRNA gen sequences of three strains: CD4 (type D) isolated from the North, DKH (type D) from Khanh Hoa va CV58 (type A) from Ninh Thuan were totally homologue (100 percent), demonstrated that these gens were highly conserve and homologue among C. perfringens strains of different toxinotypes and different isolated places
among the other strains (CV58, CV135 and type C), they differed by 1 nucleotide, or level of similarity 99.8 percent. This difference could be a random point mutation, these results suggest that the 645bp fragments on 16S rRNA of 5 C. perfringens strains isolated from Vietnam are homologue. Phylogenetic tree analysis based on the comparison of 16S rRINA gen sequences of genus Clostridium (Collins et al., 1994
Sasaki et aI., 2001, 2002), the strains isolated from Vietnam belong to the position of genus Clostridium, Cluster l. Clade l-a, species C. perfringens.