In order to evaluate infected situation and antibiotic resistance of Salmonella spp. isolated from large scale duck farms in Can Tho city, Vietnam, an investigation was carried out on 389 samples from intestine, faeces, feed and duckpond water collected from 270 duck farms in the outskirts of the city. Results indicated that overall infected rate of Salmonella spp. in duck was 27.0 percent and in environmental materials was 9.2 percent, of which infected rate of serovar Enteritidis and Typhimurium was 5.9 percent and 19.05 percent respectively. Salmonella spp. isolates in this study were resistant to almost popularly used antibiotics, except marbofloxacine, oxytetracycline, fosfomycine, amikacine and mixture of doxycycline + neomycine.