Using antagonistic-microorganisms that capable of resistance to the pathogens for plants has been particular interesting in many laboratories in the world and Vietnam. Because they do not cause pollution. safe for people and useful animal. Within this paper the authors will give some results of isolation and selection the strains of bacteria. those capable of strong resistance to R. solanacearom, which causing bacterial wilt disease on tomato and potato plants and some results experiment in the laboratorie and greenhouse. Isolated and selected the two antagonist bacteria strains: strain H4 (Diameters of inhibite round is 25-27 mm) and D9 (Diameters of inhibite round is 18-19 mm) with strongly antagonistic activity, stability, safety biological. This strains can be used for production of antagonistic microbial product. This product was capable of limiting bacterial wilt disease on tomato and potato in greenhouse conditions at a rate of more than 70 percent.