Determination of veterinary drugs in foods of animal origin by QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).

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Tác giả: Tieyuan Chen, Huidan Deng, Yuan Gou, Huizhen He, Yi Hua, Yongli Li, Jun Liu, Xiaofang Shen, Qianran Sun, Tao Wang

Ngôn ngữ: eng

Ký hiệu phân loại: 796.068 Facilities

Thông tin xuất bản: Netherlands : Journal of chromatography. A , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 62472

A method using QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of the residues of 19 veterinary drugs in ten animal-derived matrices, including beef, pork, sheep, horse, chicken, prawn, fish, liver, milk, and fat. This method was based on the enactment of veterinary drug compounds by Korea, Canada, the United States, and the European Union in recent years. The samples were extracted using 85% acetonitrile and separated on an ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) with a gradient elution of methanol-0.2% formic acid water as the mobile phase. The detection of the analytes was achieved through the use of positive ion electrospray ionization (ESI) and multiple reaction monitoring (MRM) modes, while the quantification was conducted via the matrix-matched external standard method. Following optimization, the linearity of the target veterinary residues in the ten matrices was observed to be satisfactory, having a range of 0.5-50.0 ng/mL (R
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