Staphylococcus aureus is a cause of many different infections, including life-threatening infections. Detection of virulent genes plays an important role in diagnosis, study on pathogenesis and molecular epidemiology. The study's objective was to recognize the distribution of coagulase gene in genotypes of Staphylococcus aureus strains isolated from noses and hands of staffs working in restaurants in Hanoi. Results showed that 6 genotypes of coagulase gene were determined with one genotype predominating the other five (66.2 percent). There were different distributions of coagulase gene in S. aureus isolated from noses and hands. 91.2 percent of S. aureusharboring staffs had one genotype. 8.8 percent of S. aureus harboring staffs had 2 or 3 genotypes. In conclusion, 6 genotypes of coagulase gene were determined with genotype 6 predominating the other five (66.2 percent). 91.2 percent of S.aureus-harboring staffs had one genotype. There were possibilities of having more than one S. aureus genotype in a person.