Backgound: Laparoscopic cholecystectomy is a golden standard in the procedure of choice in all the gallbladder diseases at hospitals. Single-port laparoscopic cholecystectomy was applied in some hospitals but they had to be used a specified port and laparoscopic instruments. The author report early results in laparoscopic cholecystectomy using two trocars with a single-incision perumbilical with common laparoscopic instruments. Method: From August 2010 to April 2011, the author performed 17 two-port single incision laparoscopic cholecystectomy perumbilical. In technique, after establishing umbilical carbon dioxide pneumoperitoneum per 10 mm trocar, a 45° scope was inserted, and a second 5-mm trocar was positioned to the right of this trocar. Then two stitches with absorbable sutures were passed: one at the fundus to pull up the gallbladder, and the second through the neck of the gallbladder to expose the structure of the Calot triangle. Cholecystectomy was performed and the gallbladder was extracted through the umbilical port. Results: Seventeen cases of two-trocar single-incision laparoscopic cholecystectomy perumbilical have been performed. The median age was 49 years (range, 27- 79). The average operating time was 60 minutes (range, 30-150) and the median hospital stay was 2 days. Two cases were additional trocar 5mm to stop bleeding the gallbladder bed and drainage. There was no case conversion open. There have been no deaths or complications during or after surgery. During 1 week to 1 month follow up, no problems related to the cholecystectomy have been reported. Conclusion: Two-port single incision cholecystectomy is technically feasible, safety and may further improve the surgical outcomes ill terms of postoperative pain and cosmesis. It can be considered for routine practice by surgeons who are familiar with the two-port laparoscopic surgery technique.