The 04 strains of virus infected in neurvous central system namely A-HUI01, A-HUl06, A-HUI12 and A-HUI17 were detected from 30 samples of Anguilla anguilla collected in Central of Vietnam, these strains also can be innoculated to GS cells. By nucleotide sequencing method to identify sequence of variable region T4 belong to RNA2, all of four this trains were identical in European nervous necrosis vims genotype.