A suitable protocol for meristem-ti culture for garlic (Allium sativum L.) to eliminate viruses was studied and established. The presence of viruses were identified by: (i) mechanical inoculation of inoculum isolated from infected garlic plants on indicator Chenopodium album L. and (ii) RT-PCR using potyvirus degenerate primers. After pretreatment with ethanol (70 percent) for 1 minute, shoots from infected plants were further treated with sodium dichloroisocyanurate (5g/l) for 5 minutes to eliminate microorganisms. Under a microscope, leaves of infected plants were removed one-by-one and meristems were excised and grown on MS+30g saccarose + 1 mg BA/l to produce shoots. It is indicated that to get completely virus-free plants, meristems shoud be excised with size below 0.3 mm in length. If the exised meristem explants are of 1mm in size the culture medium should be added with ribavirin (20mg/l). The virus-free shoots that were multiplied on MS + 30g saccarose + 0.5mg alphaNAA/l + 2.0mg BA/l media yield not only the highest regeneration rate (4.08 times) but also the best growth (7.55cm). Adventitious shoots can also be regenerated via calluses on MS + 15g saccarose/l + 10g glucose/l + 5g manltol/l + 2.0mg BA/l media. The best medium for root generation was MS + 30g saccarose + 0.5g activated charcoal/l + 0.5mg alphaNAA/l, on which, 100 percent shoots produced adventitous roots (5.19 roots per shoot on avarage) and the resulting plants grew normally.