The orchid Dendrobium fimbriatum Hook (Hoang Thao Long Nhan), a beautiful orchid species, which is currently used for house decoration as well as medicine, is in danger of being extinction. In vitro studies of orchid Dendrobium fimbriatum Hook. (Hoang Thao Long Nhan) were conducted in order to conserve and increase the genetic pool of this precious orchid species. The results showed that the suitable materials used for micropropagation were 3 months old fruits. Most suitable medium for germination and protocorm formation was MS+100ml ND+10g saccharose + 6.0g agar/liter. The optimum medium for propagation of protocorms was KC +100ml ND+10g saccharose + 60g potato + 6.0g agar/liter. The most appropriate medium for fast in vitro propagation of shoots was MS+100ml ND+20g saccharose + 60g ripe banana + 6.0g agar/liter. The medium used for growing of complete plants was RE +10g sacharose +1g THT + 6.0g agar/liter.