Hybrid mesoporous SBA-15 and SBA-16 materials were synthesized via hydrothermal treatment with a non-ionnic triblock copolyme P123 (SBA-15) and Fl27 (SBA-16) as the templates. SBA-15 and SBA-16 were functionalized with 3-aminopropyltriethoxysilane (APTES) and then activated by glutardialdehyd. The obtained samples were characterized by different techniques such as XRD, BET, IR and DTA. It is showed that both SBA-15 and SBA-16 samples have an highly ordered nanopore structure and the amin functional groups were grafted onto the surface of SBA-15 and SBA-16 sample. Although SBA-16 has a larger surface area than SBA-15, it has less binding capacity with APTES and glutadiandehyd. This influence can be explained by bottle-shaped structure with a narrow window size of SBA-16 that has hindered the modified surface compared with SBA-15 material.