A long-term Chloroquine usage was attributable the appearance of chloroquin-resistant P. falciparum. However, transfer from Lysin to Theronin (K76T) was suggested to reduce the chloroquin resistance or re-increase efficacy of treatment. This study aimed to determine the mutation location at 76, in the gene Pfcrt: codon 76, mutation K76T. Nested PCR study of 31 samples revealed 26 Pfalciparum, 3 P. vivax and 2 mixed infection with both P. falciparum and P. vivax. Analysis of chloroquin resistance mutation Pfcrt at the location 76 showed that 9 of 26 samples P. falciparum were still sensitive (34.6 percent) and 17 other were resistant to chloroquine (65.4 percent). The above mentioned results have preliminarily contributed to the evaluation of chloroquine resistant P. falciparum in the hyper endemic areas of Vietnam.