In this experiment, the author examined the proportion of groups of different sized follicles on the surface of pig ovaries and evaluate the effects of follicle size to quality oocytes during in vitro culture. On ovaries, the author are divided into 3 groups of different sized follicles under 2mm, 2-3mm and 3mm the results is (60.8 + or - 3.1 percent and 26.1 + or - 2.0 percent and 13.1 + or - 2.5 percent, respectively) (P 0.05). The rate of grade A oocytes in groups of 3 mm follicles than group 2-3mm follicles (20.5 + or - 6.8 percent and 5.8 + or - 2.3 percent), while less than 2 mm without the oocyte capsule type A (0 percent) (P 0.05). Oocytes grade B from group 2-3mm and 3mm similar rate (63.1 + or - 4.9 percent and 62.5 + or - 7.8 percent) is less than 2 mm follicles in the lower group was only 40,5 + or - 4.1 percent (P 0.05). The oocytes in the group qualify conducted follicles cultured in 38.5°C and 5 percent CO2 and 95 percent N. The results showed that the rate of oocyte maturation in the highest group 3mm is 60.7 + or - 5,4 percent followed by group 2-3mm follicles for maturation rate is 29.7 + or - 4.6 percent and less than 2 mm follicle group is 6.8 + or - 3.1 percent (P 0.05). Follicle size affects the results obtained and the rate of oocyte maturation in vitro culture process. The follicles size 3 mm for best results both in terms of good oocytes and oocyte maturation rate after the in vitro maturation.