Biomass technology of plant cells based on cellular differentiation has been widely applied in many fields such as pharmaceuticals, functional foods, food additives, agricultural and forestry. It both made materials for production and conserved plant genetic resources. A larger number of plant-derived active ingredients were produced by biomass technology. Ginseng products made from in vitro ginseng roots containing saponin are very popular worldwide. Many studies have been conducted to produce ginseng root biomass from adventitious and secondary roots. In this study, the formation of adventitious roots of Vietnamese ginseng (Panax vietnamensis) from in vitro calli and leaf tissues were compared. Culture media was also optimized for producing root biomass in bioreactor. After eight weeks of culture, all leaf explants on SH media supplemented with 3 mg/l NAA, 30 g/l sucrose, and 8 g/l agar produced high adventitious roots with an average number of 24.73 roots per explant. These adventitious roots could be used for rapid multiplication in bioreactor systems for extracting saponins in commercial purposes. The regeneration system of adventitious roots from callus and in vitro leaf could be applied for transformation study of Panax vietnamensis.