Today, blood transfusion safety is a key task of the blood transfusion industry. Safety on the immune increasingly valued, the deployment of some immunological tests before transfusion to ensure safe blood transfusion is urgent. Viet-Tiep Friendship Hospital has developed screening and identification of abnormal antibody from January 2011. The authors studied screening and identifying irregular antibodies in 544 patients had transfused red blood cell mass, we see results: Detection 3 per 544 patients reporting reactions harmony using AHG (+) accounted for 0.55 percent. There are 02 patients with negative results at 22°C (under normal conditions) but the positive with AHG use up accounted for 0.37 percent. In patients with multiple-blood transfusions, percentage of positive responses harmony AHG using 5 times compared to cross-react normally. Among 03 positive cases were anti-E antibody, anti-E and c and anti-Mia,