Five flavonoid glycosides, phellamurin (1), epimedoside C (2), astragalin (3), nicotiflorin (4), and trifolin (5) were isolated from the methanol extract of the Tetradium glabrifolium leaves by various chromatographic experiments. The structure elucidation of isolated compounds was done by spectroscopic methods including ESI-MS, 1D- and 2D-NMR in comparison with the literature values. This is the first report of these compounds from T. glabrifolium.