After the survey and collection of samples of fish belonging to the suborder Gobioidei in Tra Khuc river, the authors have listed 18 species belonging to 11 genus and 2 families, among which the Gobiidae is the most plentiful with 14 species. Among the species defined, there are 5 species with high economic value. In comparison with the previous studies by Nguyen Huu Duc in 1995, the authors have added 15 species to the fish catalog belonging to the suborder Gobioidei in Tra Khuc river, Quang Ngai province.