Fecal samples (n=378) were collected from children less than 5 years of age admitted to Thai Binh pediatric hospital for acute gastroenteritis from September 2010 throught August 2011 for assessing thethe prevalence and characterization of different diarrheal pathogens. Prevalences of rotavirus (RV), norovirus (NV), adenovirus, astrovirus and DEC in fecal samples in Thai Binh children 36.2 percent, 32.8 percent, 5.6 percent, 1.1 percent and 17.5 percent, respectively emphasizing the important role of both RV and NV in children's acute diarrhea. The RV-G3 genotype, used to prevailed during 2006-2009 now represented only 13 percent oftotal samples, whereas the RV-G1P[8] became the dominant type indicating the cyclic changing pattern of RV genotypes. Other genotypes including G2P[4], G4P[6] or mixed P[6] and P[9] were also detected. The NV GII.3 and GII.4 (Minerva and Apeldoorn-like) genotypes represent 27.8 percent and 66 percent of circulating strain, respectively while other genotypes GII.2, GII.7, GII.12 and GII13 and GI8 were also identified. The common DEC pa.thotypes such as EAEC, EPEC, ETEC, EIEC were all detected with detection rates of 10.3 percent, 5 percent, 5 percent and 2 percent of total samplessamples, respectively. This study confirms the important role of NV and RV in children's diarrhea, and underscores the need for continuing assessment of both composition of diarrheal pathogens and the strain distributions before and after mass vaccination to assess the vaccine impact and suggest additional diarrhea control measure after RV vaccine.