Xuan Son National Park is one of 30 National Parks on the territory of Vietnam, of two on six level classifications of protected areas of the world. Xuan Son National Park is considered as a forest, which has rich biodiversity, diversity of terrain construct that make diverse landscape. Specially, this is the only National Park with forest on limestone mountains in the country. To evaluate the diversity as well as the conservation of biodiversity of flora, 3 transects survey with 9 quadrats, divided into 45 plots have been established. A statistical research on Xuan Son National Park had pointed that this park has 7 major ecosystems with 6 branches, 1,217 species, 680 plants is divided into four basic forms of life, including 40 species listed in the Vietnam Red Book and added some new species for the flora of Vietnam. Simultaneously determine the big value of using of 1,171 over 1,217 plant species, accounted for 96.22 percent as well as the opportunities and challenges in the conservation of diversity in Xuan Son National Park. From the results, building the propose solutions to contribute to conservation of flora diversity and abundance of capital Xuan Son National Park and future research on the relationship between environmental factores with species distribution.