Background: Patients's opinion regarding health care quality has acquired great importance. Patient's satisfaction play an increasingly important role in quanlity of care reforms and health-care delivery. The study aimed to measure patient satisfaction at clinical departments in order to know the patients' perspectives and expectation of the services and make appropriate improvements accordingly. Methods: This was a cross-sectional study conducted at National Institute of Hematology and Blood Transfusion, for a period from September to December 2011. Sample size was 390 In-patients. Results: Among the participants, overall satisfaction rate was equal to 67.2 percent. Satisfaction rate of communication skill of health workers was 81.6 percent (sub-categories ranging from 72 percent to 98.2 percent), satisfied with care service was 78 percent (74.6 percent to 95.1 percent), satisfied with waiting time to access health care services was 66.8 percent (71.5 percent to 89.2 percent) and satisfied with facilities/services was 58.8 percent (62.2 percent to 79.2 percent). Age, marital status, demographics (urban/rural), educational level of patients was significantly associated with satisfaction level.