Screening for transfusion-transmissible infections (TTls) to exclude blood donations at risk of transmitting infection from donors to recipients is a critical part of the process of ensuring that transfusion is as safe as possible. Effective screening for evidence of the presence of the most common and dangerous TTls can reduce the risk of transmission to very low levels. Objectives: To evaluate the prevalence of markers of hepatitis B surface antigen (HBsAg) and antibodys hepatitis C virus (anti HCV) and antigens and antibodies Human immunodeficiency virus (HIV AgAb) and Syphilis of blood donors in National Institute of Hematology and Blood Transfusion for three years 20092011. Materials: The serum or plasma of blood donors were screened HBsAg, anti HCV, HIVAgAb and Syphilis of NIHBT from 1/1/2009 to 31/12/2011. Methods: Statistic, description. Results: The results of screening tests 345 925 units of blood donor NIHBT (2009-2011) for HBsAg, antiHCV, HIVAgAb and Syphilis are 0.99 percent, 0.16 percent, 0.02 percent and 0:08 percent. Conclusions: Ratio HBsAg, antibody HCV, a,ntigens-antibody HIV, Syphilis of blood donors (2009-2011) of NIHBT are 0.99 percent, 0.16 percent, 0.02 percent and 0.08 percent. Ratio HBsAg, antibody HCV, antigens-antibody HIV, Syphilis of volunteer donors are 1.01 percent, 0.16 percent, 0.02 percent, 0.08 percent. Ratio. HBsAg, antibody HCV, antigens-antibody HIV, Syphilis of paid donors are 0.56 percent, 0.22 percent, 0.02 percent, 0.07 percent.