The sewage water contains many pollutants having high concentration, especially ammonia and orthophosphate which they contribute environment pollution therefore they need to be treated before releasing to environment. The heterotrophic nitrogen removal bateria Pseudomonas stutzeri and polyphosphate-accumulating bacteria Bacillus subtilis used in this experiment to remove nitrogen and orthophosphate in sewage water in bioreactor 1 L. The results showed that D3b strain has the highest nitrogen removal ability, DIT.001 L strain is good poly-P bacteria. In the aerobic condition, the mixture of two these strains removed 99.8 percent ammonia after 48 hours and changed orthophosphate to poly-P (68.7 percent) after 24 hours for sewage water treatment. Supplemented of glucose as carbon source into sewage water, both of strains decreased 96.01 percent ammonia concentration, 76.04 percent orthophosphate and the lowest released ammoniac concentration (202.33 mg/l) in the sewage water after 36 hours in bioreactor 1 L. In bioreactor 10 L., the mixture of two strain plus glucose (5 g/l) reduced 90.83 percent ammonia, 73.01 percent orthophosphate with the lowest released ammonic concentration (114.85 mg/l) in sewage water treatment process however nitrogen total, phosphate total and chemical oxy demand (COD) concentration in the sewage water reduced 63.08 percent, 35.48 percent and 25.16 percent, respectively but these results were higher than standard of QCVN24 and 25:2009/BTNMT.