Microsatellites are widely used for population genetics purposes, especially when the scope of the study involves comparing closely related individuals. The development of microsatellite marker becomes a challenge in many fields of study including mollusk for various reasons related to the cost for the marker isolation. Cross-amplification of microsatellite marker becomes useful method that can'save time, cost and help to underst DNA the relationship between the investigated species. In this study, three microsatellie loci (Kaki12, Kaki18, va Kakicgi2) isolated from Pacific oyster (Crassostrea gigas) have been tested for six bivalves species, including Crassostrea rivularis, Meretrix Iyrata, Austriella corrugate, Lutraria philipinarum, Anadara granosa and Semipallium fulvicostatum. Five samples of each species were collected and DNA was extracted from muscle tissue. After that, it was amplified by PCR with three primers Kaki12, Kaki18 and Kakicgi2. Result on electrophoresis of PCR products on 1 percent agarose gel showed that, the amplification of Kaki12, Kaki18 DNA Kakicgi2 on Crassostrea rivularis was successes. Although the PCR products appear for Kakicgi2 on Anadara granosa and Semipallium fulvicostatum, it was not clear. In case of other samples, there was no PCR product on the gel except Kaki18 for Anadara granos and Semipallium fulvicostatum. The success of cross-species amplification suggests that, these microsatellites should prove useful for studies in population genetics, pedigree analysis, gene mapping as well as the relationships between species of Anadara granos, Semipallium fulvicostatum and Crassostrea gigas. However, studies on different microsatellite markers for different species should be continued for the useful information on molecular genetics in many aspects.