Bulb samples were sterilized mainly by HgCl2 and javen in different experiments of concentration and time. After sterilized, the samples were cultured in bud creation medium (medium was added with different mount of saccharose and BAP) and in callus creation medium adding different concentration of auxin. The result showed 3 main conclusions. The first, best tuberose sterilized regulation is 0.2 percent of HgCl2 in 15 minutes combined with 15 percent javen in 10 minutes. The second, medium, added with 30g/l of saccharose and 5mg/l BAP is the most suitable for direct bud regeneration from sleep node. The last, medium with 1.5 mg/ml of 2.4D is the most suitable for callus creation from bulb tuberose samples.