White spot syndrome virus is very popular in shrimp. It is a dangerous disease. Some studies reported that protein VP28 is one of proteins acting as a key factor to infect the shrimp. When the virus infect into shrimps these proteins will be expression and shrimps will be die. This article reported an immunochromatography device based on the principle of sandwich assay (lateral flow test). This test were developed by using monoclonal anti - VP28 antibodies which produced by Institute of Biotechnology. The protein VP28 in the shrimp was captured by a monoclonal anti-VP28 antibody gold conjugated in the conjugate pad. This complex will was captured by other monoclonal anti -VP28 coated on the nitrocellulose membrane, so T line appear. When an amount of the extracted sample was applied to the sample pad, the solution migrates by capillary action through the test strip. If it was negative (without VP28 protein in sample), the T line disappeared. If it was positive (with VP28 protein), the T line developed in the T region. This method had high sensitivity and specificity. Result was read rapidly in 15 minutes. The authors chose several appropriate conditions as: concentration of capture reagents on test line and control line, concentration of colloidal conjugated gold on the conjugate pad, treated sample pad, sample extract buffer to make a rapid test. The authors successfully created a rapid test to detect WSSV in shrimp. The limit of detection of VP28 is 5 ng/ml. Reading results are set at 15 minutes. The authors also make a sample extractor which is simple and activites in the field. Store the test below room temperature.