Circumsporozoite protein (CSP) is the primary component of two malaria vaccines that have been validated by World Health Organisation. However, these vaccines do not include antigens from non-falciparum species, which should also be considered. The csp gene was amplified and sequenced in 14 Plasmodium ovale cuitisi (Poc) isolates from Cameroon. The C and N-terminal regions showed perfect conservation, while the central region showed repeated motifs of nine amino acids. Interestingly, in addition to nano-repeat motifs reported in the literature, a new motif (QSEGNPPAP) was observed in four samples, resulting in haplotype combinations that can affect the antigenicity of protein and therefore should be taken into account when designing a CSP-based vaccine against Poc malaria.