This study examines the consistent and inheritance of microsatellite sex linked markers in the second generation of tilapia. Alleles linked to sex of micro satellite markers namely GM354, UNH104 and UNH995 were tested on brood stock of Oreochromis aurreus and Oreochromis niloticus that aims to produce allmale hybrid tilapia in progeny. The number of alleles observed was 3-6 alleles/locus, in addition the PIC was approximately 0.494-0.820. All studied microsatellite markers exhibited not significant deviation from Hardy-Weinberg equilibrium (P0.001). The frequency of specific alleles at each micro satellite loci in the two tilapia species ranged from 40 percent to 89 percent. On average, homogeneous genotype is similar between the two tialapia species (37.0 percent and 38.5 percent) (P0.05). The specific alleles were existed of high frequency and constant inheritance of specific alleles in the second generation of tilapia suggest to be applicable for selection of parents so as to produce high pecentage of all-male in off spring.